%0 Journal Article %T Chronic TNFalpha and cAMP pre-treatment of human adipocytes alter HSL, ATGL and perilipin to regulate basal and stimulated lipolysis. %+ Institut de médecine moléculaire de Rangueil (I2MR) %+ Laboratoire de Biochimie [CHU Toulouse] %A Bézaire, Véronic %A Mairal, Aline %A Anesia, Rodica %A Lefort, Corinne %A Langin, Dominique %< avec comité de lecture %@ 0014-5793 %J FEBS Letters %I Wiley %V 583 %N 18 %P 3045-9 %8 2009-09-17 %D 2009 %R 10.1016/j.febslet.2009.08.019 %M 19695247 %Z Life Sciences [q-bio]/Biochemistry, Molecular BiologyJournal articles %X We examined the effects of chronic TNFalpha and dibutyryl-cAMP (Db-cAMP) pre-treatment on the lipolytic machinery of human hMADS adipocytes. TNFalpha decreased adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL) protein content and triglycerides (TG)-hydrolase activity but increased basal lipolysis due to a marked reduction in perilipin (PLIN) protein content. Conversely, Db-cAMP increased ATGL and HSL protein content but prevented PLIN phosphorylation, the net result being accentuated basal lipolysis. In forskolin-stimulated conditions, TNFalpha and Db-cAMP pre-treatment decreased stimulated TG-hydrolase activity and impaired PLIN phosphorylation. Together, this resulted in a severely attenuated response to forskolin-stimulated lipolysis. %G English %L inserm-00419424 %U https://inserm.hal.science/inserm-00419424 %~ INSERM %~ UNIV-TLSE3 %~ UNIV-UT3 %~ UT3-INP %~ UT3-TOULOUSEINP