%0 Journal Article %T Developmentally regulated association of plastid division protein FtsZ1 with thylakoid membranes in Arabidopsis thaliana. %+ Plastes et différenciation cellulaire (PDC) %+ Centre de Recherches sur les Macromolécules Végétales (CERMAV) %+ ANTE-INSERM U836, équipe 4, Muscles et pathologies %A El-Kafafi, El-Sayed %A Karamoko, Mohamed %A Pignot-Paintrand, Isabelle %A Grunwald, Didier %A Mandaron, Paul %A Lerbs-Mache, Silva %A Falconet, Denis %Z This work was supported by a grant [ACIDRAB (ActionConcertée Incitative-Dynamique et Réactivité des Assemblages Biologiques) 03/41, number 03 5 90 to D. F. and number 03 5 92 to I.P.-P.] from the CNRS and the MEN (Ministère de l'Education Nationale), by the Egyptian government (fellowship toE.-S. E.-K.), and by the Ivory Coast government (fellowship to M.K.). %< avec comité de lecture %@ 0264-6021 %J Biochemical Journal %I Portland Press %V 409 %N 1 %P 87-94 %8 2008-01-01 %D 2008 %R 10.1042/BJ20070543 %M 17725544 %K Arabidopsis thaliana %K chloroplast division %K FtsZ gene family %K plastid division %K plastid localization %K thylakoid membrane %Z Life Sciences [q-bio]/Cellular BiologyJournal articles %X FtsZ is a key protein involved in bacterial and organellar division. Bacteria have only one ftsZ gene, while chlorophytes (higher plants and green alga) have two distinct FtsZ gene families, named FtsZ1 and FtsZ2. This raises the question of why chloroplasts in these organisms need distinct FtsZ proteins to divide. In order to unravel new functions associated with FtsZ proteins, we have identified and characterized an Arabidopsis thaliana FtsZ1 loss-of-function mutant. ftsZ1-knockout mutants are impeded in chloroplast division, and division is restored when FtsZ1 is expressed at a low level. FtsZ1-overexpressing plants show a drastic inhibition of chloroplast division. Chloroplast morphology is altered in ftsZ1, with chloroplasts having abnormalities in the thylakoid membrane network. Overexpression of FtsZ1 also induced defects in thylakoid organization with an increased network of twisting thylakoids and larger grana. We show that FtsZ1, in addition to being present in the stroma, is tightly associated with the thylakoid fraction. This association is developmentally regulated since FtsZ1 is found in the thylakoid fraction of young developing plant leaves but not in mature and old plant leaves. Our results suggest that plastid division protein FtsZ1 may have a function during leaf development in thylakoid organization, thus highlighting new functions for green plastid FtsZ. %G English %2 https://inserm.hal.science/inserm-00413580/document %2 https://inserm.hal.science/inserm-00413580/file/El_Kafafi_et_al.pdf %L inserm-00413580 %U https://inserm.hal.science/inserm-00413580 %~ INSERM %~ CEA %~ UGA %~ CNRS %~ UNIV-GRENOBLE1 %~ U836 %~ U873 %~ CERMAV %~ INC-CNRS %~ TEST2-HALCNRS