%0 Journal Article %T Cytokines correlate with age in healthy volunteers, dialysis patients and kidney-transplant patients. %+ Institut de médecine moléculaire de Rangueil (I2MR) %+ Gambro Dialysatoren GmbH %+ Service de Néphrologie - Hypertension Artérielle Dialyse - Transplantation %A Böhler, Torsten %A Canivet, Cindy %A Nguyen, Phuong Ngan Le %A Galvani, Sylvain %A Thomsen, Mogens %A Durand, D. %A Salvayre, Robert %A Nègre-Salvayre, Anne %A Rostaing, Lionel %A Kamar, Nassim %< avec comité de lecture %@ 1043-4666 %J Cytokine %I Elsevier %V 45 %N 3 %P 169-73 %8 2009-03 %D 2009 %R 10.1016/j.cyto.2008.11.014 %M 19147373Journal articles %X T-cell functions are currently used as biomarkers for the pharmacodynamic monitoring of immunosuppressive drugs or as disease biomarkers of inflammation/sepsis and organ rejection. In order to evaluate co-factors potentially influencing the expression of the immunological biomarkers, we explored T-cell proliferation, T-cell activation (CD25 and CD71 expressions) and intra-lymphocyte cytokine production (interleukin (IL)-2 and tumor necrosis factor (TNF)-alpha) in healthy volunteers, dialysis patients and stable kidney-transplant patients treated with standard immunosuppressive therapy, i.e. tacrolimus, mycophenolic acid with or without steroids. Age was positively correlated with TNF-alpha expression in all three patient populations, and with IL-2 expression in healthy volunteers and kidney-transplant patients. Further age was correlated with inhibition of lymphocyte proliferation in healthy volunteers and with the T-cell activation marker CD25 in kidney-transplant patients. In healthy volunteers lymphocyte proliferation was higher in woman as compared to men. Other biomarkers of T-cell function were independent of the gender. In the kidney-transplant patient group a significantly lower expression of all biomarkers of T-cell functions compared to healthy volunteers and dialysis patients. In dialysis patients we found significant increased IL-2 expression compared to healthy volunteers, while the other T-cell functions were not significantly different. Further time on dialysis had no effect on the level of biomarker expression. In conclusion we found decreased T-cell functions in kidney-transplant patients compared to healthy volunteers and dialysis patients, increased IL-2 expression in dialysis patients compared to healthy volunteers and in all three populations we found a correlation of age and intra-T-lymphocyte TNF-alpha expression. %G English %L inserm-00410387 %U https://inserm.hal.science/inserm-00410387 %~ INSERM %~ UNIV-TLSE3 %~ UNIV-UT3 %~ UT3-INP %~ UT3-TOULOUSEINP