%0 Journal Article %T Comparative toxicity of 24 manufactured nanoparticles in human alveolar epithelial and macrophage cell lines. %+ Physiopathologie et Epidemiologie de l'Insuffisance Respiratoire %+ Institut National de l'Environnement Industriel et des Risques (INERIS) %+ Laboratory of Pneumology %+ CIC - CHU Bichat %A Lanone, Sophie %A Rogerieux, Françoise %A Geys, Jorina %A Dupont, Aurélie %A Maillot-Marechal, Emmanuelle %A Boczkowski, Jorge %A Lacroix, Ghislaine %A Hoet, Peter %< avec comité de lecture %J Part Fibre Toxicol %V 6 %P 14 %8 2009 %D 2009 %R 10.1186/1743-8977-6-14 %M 19405955 %Z Life Sciences [q-bio]/Biochemistry, Molecular BiologyJournal articles %X ABSTRACT: BACKGROUND: A critical issue with nanomaterials is the clear understanding of their potential toxicity. We evaluated the toxic effect of 24 nanoparticles of similar equivalent spherical diameter and various elemental compositions on 2 human pulmonary cell lines: A549 and THP-1. A secondary aim was to elaborate a generic experimental set-up that would allow the rapid screening of cytotoxic effect of nanoparticles. We therefore compared 2 cytotoxicity assays (MTT and Neutral Red) and analyzed 2 time points (3 and 24 hours) for each cell type and nanoparticle. When possible, TC50 (Toxic Concentration 50 i.e. nanoparticle concentration inducing 50% cell mortality) was calculated. RESULTS: The use of MTT assay on THP-1 cells exposed for 24 hours appears to be the most sensitive experimental design to assess the cytotoxic effect of one nanoparticle. With this experimental set-up, Copper- and Zinc-based nanoparticles appear to be the most toxic. Titania, Alumina, Ceria and Zirconia-based nanoparticles show moderate toxicity, and no toxicity was observed for Tungsten Carbide. No correlation between cytotoxicity and equivalent spherical diameter or specific surface area was found. CONCLUSION: Our study clearly highlights the difference of sensitivity between cell types and cytotoxicity assays that has to be carefully taken into account when assessing nanoparticles toxicity. %G English %2 https://inserm.hal.science/inserm-00407214/document %2 https://inserm.hal.science/inserm-00407214/file/1743-8977-6-14.pdf %L inserm-00407214 %U https://inserm.hal.science/inserm-00407214 %~ INSERM %~ INERIS %~ UNIV-PARIS7 %~ BICHAT %~ UNIV-PARIS