Ablation of PRC1 by small interfering RNA demonstrates that cytokinetic abscission requires a central spindle bundle in mammalian cells, whereas completion of furrowing does not. - Inserm - Institut national de la santé et de la recherche médicale Access content directly
Journal Articles Molecular Biology of the Cell Year : 2005

Ablation of PRC1 by small interfering RNA demonstrates that cytokinetic abscission requires a central spindle bundle in mammalian cells, whereas completion of furrowing does not.

Abstract

The temporal and spatial regulation of cytokinesis requires an interaction between the anaphase mitotic spindle and the cell cortex. However, the relative roles of the spindle asters or the central spindle bundle are not clear in mammalian cells. The central spindle normally serves as a platform to localize key regulators of cell cleavage, including passenger proteins. Using time-lapse and immunofluorescence analysis, we have addressed the consequences of eliminating the central spindle by ablation of PRC1, a microtubule bundling protein that is critical to the formation of the central spindle. Without a central spindle, the asters guide the equatorial cortical accumulation of anillin and actin, and of the passenger proteins, which organize into a subcortical ring in anaphase. Furrowing goes to completion, but abscission to create two daughter cells fails. We conclude the central spindle bundle is required for abscission but not for furrowing in mammalian cells.
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Dates and versions

inserm-00380211 , version 1 (14-05-2009)

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Cristiana Mollinari, Jean-Philippe Kleman, Yasmina Saoudi, Sandra A. Jablonski, Julien Perard, et al.. Ablation of PRC1 by small interfering RNA demonstrates that cytokinetic abscission requires a central spindle bundle in mammalian cells, whereas completion of furrowing does not.. Molecular Biology of the Cell, 2005, 16 (3), pp.1043-55. ⟨10.1091/mbc.E04-04-0346⟩. ⟨inserm-00380211⟩
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