%0 Journal Article %T Efficient temporally-controlled targeted mutagenesis in smooth muscle cells of the adult mouse. %+ Institut de génétique et biologie moléculaire et cellulaire (IGBMC) %+ Institut Clinique de la Souris (ICS) %A Wendling, Olivia %A Bornert, Jean-Marc %A Chambon, Pierre %A Metzger, Daniel %< avec comité de lecture %@ 1526-954X %J Genesis %I Wiley-Blackwell %V 47 %N 1 %P 14-8 %8 2009-01 %D 2009 %R 10.1002/dvg.20448 %M 18942088 %Z Life Sciences [q-bio]/Quantitative Methods [q-bio.QM]Journal articles %X To generate temporally-controlled targeted somatic mutations selectively and efficiently in smooth muscles, we have established a transgenic SMA-Cre-ER(T2) mouse line in which the expression of the Tamoxifen-dependent Cre-ER(T2) recombinase is under the control of a large genomic DNA segment of the mouse smooth muscle alpha actin (SMA) gene, contained in a Bacterial artificial chromosome (Bac). In this transgenic mouse line, Cre-ER(T2)-mediated recombination of LoxP-flanked target DNA is strictly Tamoxifen-dependent, and efficient in both vascular and visceral smooth muscle cells. Moreover, with the exception of few cardiomyocytes, LoxP-flanked DNA excision is restricted to smooth muscle cells. Thus, SMA-Cre-ER(T2) mice should be of great value to analyze gene function in smooth muscles, and to establish new animal models of human smooth muscle disorders. %G English %L inserm-00357169 %U https://inserm.hal.science/inserm-00357169 %~ INSERM %~ CNRS %~ UNIV-STRASBG1 %~ IGBMC %~ UNIV-STRASBG %~ SITE-ALSACE