%0 Journal Article %T Identification and characterization of a transmembrane isoform of CD160 (CD160-TM), a unique activating receptor selectively expressed upon human NK cell activation. %T CD160-TM, an unique NK cell activating receptor %+ Institut Mondor de recherche biomédicale (IMRB) %A Giustiniani, Jérôme %A Bensussan, Armand %A Marie-Cardine, Anne %Z This work was supported by the Institut National de la Santé et de la Recherche Médicale (INSERM) and Paris XII University. %< avec comité de lecture %@ 0022-1767 %J Journal of Immunology %I Publisher : Baltimore : Williams & Wilkins, c1950-. Latest Publisher : Bethesda, MD : American Association of Immunologists %V 182 %N 1 %P 63-71 %8 2009-01-01 %D 2009 %M 19109136 %K Natural Killer Cells %K Cell Surface Molecules %K Cytotoxicity %K Signal Transduction %Z Life Sciences [q-bio]/Biochemistry, Molecular BiologyJournal articles %X CD160 has been initially identified as a GPI-anchored MHC-class I activating receptor mainly expressed on peripheral blood NK cells. Herein, we report the identification of three additional CD160-related mRNAs generated through alternative splicings of the CD160 gene, among which one encoded a putative CD160 transmembrane isoform (CD160-TM). We first establish that CD160-TM surface expression is highly restricted to NK cells and is activation-dependent. Additionally, we provide evidence that CD160-TM represents a novel activating receptor, as assessed by the increased CD107a NK cell surface mobilization observed upon its engagement. Finally, we demonstrate that the CD160-TM cytoplasmic tail is by itself sufficient to mediate the recruitment of Erk1/2 signaling pathway, and that the initiation of this activation process is dependent on the Src-family kinase p56(lck). The identification of CD160-TM therefore provides new possibilities regarding the role of CD160 isoforms in the regulation of NK cell functions. %G English %2 https://inserm.hal.science/inserm-00352331/document %2 https://inserm.hal.science/inserm-00352331/file/Table_I.pdf %2 https://inserm.hal.science/inserm-00352331/file/Figures_1-6.pdf %2 https://inserm.hal.science/inserm-00352331/file/Giustiniani_et_al_revised_.pdf %2 https://inserm.hal.science/inserm-00352331/file/inserm-00352331_edited.pdf %L inserm-00352331 %U https://inserm.hal.science/inserm-00352331 %~ INSERM %~ IMRB %~ UPEC