CD4-CCR5 interaction in intracellular compartments contributes to receptor expression at the cell surface. - Inserm - Institut national de la santé et de la recherche médicale Access content directly
Journal Articles Blood Year : 2009

CD4-CCR5 interaction in intracellular compartments contributes to receptor expression at the cell surface.

Lamia Achour
  • Function : Author
Mark G.H. Scott
  • Function : Author
Alain Thuret
  • Function : Author
Georges Bismuth
  • Function : Author
Stefano Marullo
  • Function : Correspondent author
  • PersonId : 842931

Connectez-vous pour contacter l'auteur

Abstract

The association of CD4, a glycoprotein involved in T-cell development and antigen recognition, and CC chemokine receptor 5 (CCR5), a chemotactic G protein-coupled receptor, which regulates trafficking and effector functions of immune cells, forms the main receptor for HIV. We observed that the majority of CCR5 is maintained within the intracellular compartments of primary T lymphocytes and in a monocytic cell line, contrasting with its relatively low density at the cell surface. The CCR5-CD4 association, which occurs in the endoplasmic reticulum, enhanced CCR5 export to the plasma membrane in a concentration-dependent manner, whereas inhibition of endogenous CD4 with small interfering RNAs decreased cell-surface expression of endogenous CCR5. This effect was specific for CCR5, as CD4 did not affect cellular distribution of CXCR4, the other HIV coreceptor. These results reveal a previously unappreciated role of CD4, which contributes to regulating CCR5 export to the plasma membrane.
Fichier principal
Vignette du fichier
inserm-00346678_edited.pdf (1.26 Mo) Télécharger le fichier
Achour_et_al.pdf (3.82 Mo) Télécharger le fichier
Origin : Files produced by the author(s)
Origin : Files produced by the author(s)
Loading...

Dates and versions

inserm-00346678 , version 1 (17-12-2008)

Identifiers

Cite

Lamia Achour, Mark G.H. Scott, Hamasseh Shirvani, Alain Thuret, Georges Bismuth, et al.. CD4-CCR5 interaction in intracellular compartments contributes to receptor expression at the cell surface.. Blood, 2009, 113 (9), pp.1938-47. ⟨10.1182/blood-2008-02-141275⟩. ⟨inserm-00346678⟩
132 View
646 Download

Altmetric

Share

Gmail Facebook X LinkedIn More