%0 Journal Article %T Weak but uniform enrichment of the histone variant macroH2A1 along the inactive X chromosome. %+ Institut d'oncologie/développement Albert Bonniot de Grenoble (INSERM U823) %+ Research Institute of Molecular Pathology (IMP) %+ Institut Jacques Monod (IJM (UMR_7592)) %+ Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] (IRI) %A Mietton, Flore %A Sengupta, Aditya, K. %A Molla, Annie %A Picchi, Gisele %A Barral, Sophie %A Heliot, Laurent %A Grange, Thierry %A Wutz, Anton %A Dimitrov, Stefan %Z INSERM, CNRS, ANR, GEN-AU %< avec comité de lecture %@ 0270-7306 %J Molecular and Cellular Biology %I American Society for Microbiology %V 29 %N 1 %P 150-6 %8 2009-01 %D 2009 %R 10.1128/MCB.00997-08 %M 18936163 %Z Life Sciences [q-bio]/GeneticsJournal articles %X We studied the enrichment and distribution of the histone variant mH2A1 in the condensed inactive X (Xi) chromosome. By using highly specific antibodies against mH2A1 and stable HEK 293 cell lines expressing either green fluorescent protein (GFP)-mH2A1 or GFP-H2A, we found that the Xi chromosome contains approximately 1.5-fold more mH2A1 than the autosomes. To determine the in vivo distribution of mH2A1 along the X chromosome, we used a native chromatin immunoprecipitation-on-chip technique. DNA isolated from mH2A1-immunoprecipitated nucleosomes from either male or female mouse liver were hybridized to tiling microarrays covering 5 kb around most promoters or the entire X chromosome. The data show that mH2A1 is uniformly distributed across the entire Xi chromosome. Interestingly, a stronger mH2A1 enrichment along the pseudoautosomal X chromosome region was observed in both sexes. Our results indicate a potential role for macroH2A in large-scale chromosome structure and genome stability. %G English %2 https://inserm.hal.science/inserm-00336261/document %2 https://inserm.hal.science/inserm-00336261/file/inserm-00336261_edited.pdf %2 https://inserm.hal.science/inserm-00336261/file/FIGURES_mH2A.pdf %2 https://inserm.hal.science/inserm-00336261/file/mH2A1_15.pdf %L inserm-00336261 %U https://inserm.hal.science/inserm-00336261 %~ INSERM %~ UNIV-PARIS7 %~ UGA %~ CNRS %~ UNIV-GRENOBLE1 %~ IJM %~ U823 %~ UNIV-PARIS %~ UP-SCIENCES %~ TEST3-HALCNRS