Weak but uniform enrichment of the histone variant macroH2A1 along the inactive X chromosome. - Inserm - Institut national de la santé et de la recherche médicale Access content directly
Journal Articles Molecular and Cellular Biology Year : 2009

Weak but uniform enrichment of the histone variant macroH2A1 along the inactive X chromosome.

Abstract

We studied the enrichment and distribution of the histone variant mH2A1 in the condensed inactive X (Xi) chromosome. By using highly specific antibodies against mH2A1 and stable HEK 293 cell lines expressing either green fluorescent protein (GFP)-mH2A1 or GFP-H2A, we found that the Xi chromosome contains approximately 1.5-fold more mH2A1 than the autosomes. To determine the in vivo distribution of mH2A1 along the X chromosome, we used a native chromatin immunoprecipitation-on-chip technique. DNA isolated from mH2A1-immunoprecipitated nucleosomes from either male or female mouse liver were hybridized to tiling microarrays covering 5 kb around most promoters or the entire X chromosome. The data show that mH2A1 is uniformly distributed across the entire Xi chromosome. Interestingly, a stronger mH2A1 enrichment along the pseudoautosomal X chromosome region was observed in both sexes. Our results indicate a potential role for macroH2A in large-scale chromosome structure and genome stability.

Domains

Genetics
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Origin : Files produced by the author(s)
Origin : Files produced by the author(s)
Origin : Files produced by the author(s)

Dates and versions

inserm-00336261 , version 1 (03-11-2008)

Identifiers

Cite

Flore Mietton, Aditya K. Sengupta, Annie Molla, Gisele Picchi, Sophie Barral, et al.. Weak but uniform enrichment of the histone variant macroH2A1 along the inactive X chromosome.. Molecular and Cellular Biology, 2009, 29 (1), pp.150-6. ⟨10.1128/MCB.00997-08⟩. ⟨inserm-00336261⟩
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