%0 Journal Article %T Practical PCR genotyping protocols for Plasmodium vivax using Pvcs and Pvmsp1. %+ Department of Clinical Tropical Medicine [Bangkok, Thailand] (Faculty of Tropical Medicine) %+ Institut Cochin (UMR_S567 / UMR 8104) %+ Institut Cochin (IC UM3 (UMR 8104 / U1016)) %+ Wellcome Unit %+ Centre for Vaccinology and Tropical Medicine %+ Parasitologie Biomédicale %+ Parasitologie Comparée et Modèles Expérimentaux %A Imwong, Mallika %A Pukrittayakamee, Sasithon %A Grüner, Anne Charlotte %A Rénia, Laurent %A Letourneur, Frank %A Looareesuwan, Sornchai %A White, Nicholas, J. %A Snounou, Georges %Z This study was part of the Wellcome Trust-Mahidol University-Oxford Tropical Medicine Research Programme supported by the Wellcome Trust of Great Britain. %< avec comité de lecture %@ 1475-2875 %J Malaria Journal %I BioMed Central %V 4 %P 20 %8 2005 %D 2005 %R 10.1186/1475-2875-4-20 %M 15854233 %Z Life Sciences [q-bio]/Human health and pathology/Infectious diseases %Z Life Sciences [q-bio]/Microbiology and Parasitology %Z Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN]Journal articles %X BACKGROUND: Plasmodium vivax is the second most prevalent malaria parasite affecting more than 75 million people each year, mostly in South America and Asia. In addition to major morbidity this parasite is associated with relapses and a reduction in birthweight. The emergence and spread of drug resistance in Plasmodium falciparum is a major factor in the resurgence of this parasite. P. vivax resistance to drugs has more recently emerged and monitoring the situation would be helped, as for P. falciparum, by molecular methods that can be used to characterize parasites in field studies and drug efficacy trials. METHODS: Practical PCR genotyping protocols based on polymorphic loci present in two P. vivax genetic markers, Pvcs and Pvmsp1, were developed. The methodology was evaluated using 100 P. vivax isolates collected in Thailand. RESULTS AND DISCUSSION: Analysis revealed that P. vivax populations in Thailand are highly diverse genetically, with mixed genotype infections found in 26 % of the samples (average multiplicity of infection = 1.29). A large number of distinguishable alleles were found for the two markers, 23 for Pvcs and 36 for Pvmsp1. These were generally randomly distributed amongst the isolates. A total of 68 distinct genotypes could be enumerated in the 74 isolates with a multiplicity of infection of 1. CONCLUSION: These results indicate that the genotyping protocols presented can be useful in the assessment of in vivo drug efficacy clinical trials conducted in endemic areas and for epidemiological studies of P. vivax infections. %G English %2 https://inserm.hal.science/inserm-00090856/document %2 https://inserm.hal.science/inserm-00090856/file/1475-2875-4-20.pdf %L inserm-00090856 %U https://inserm.hal.science/inserm-00090856 %~ INSERM %~ PASTEUR %~ UNIV-PARIS5 %~ MNHN %~ CNRS %~ RIIP_PARIS %~ UNIV-PARIS %~ UP-SANTE %~ ALLIANCE-SU